Product Features
The assay is ready-to-use for manual methods and adaptable for analyzers. The assay is specific for NAG and has no detectable reaction with other glycosidases and has excellent sensitivity with a linear range from up to 200 U/L and excellent precision (CV <5%) . There is no significant interference from hemoglobin or albumin. Interference from bilirubin occurs only at levels higher than 5 mg/dL. The reagent is stable for 12 months from date of manufacture when stored at @ 2°-8°C.

 

 

Assay Principle
The Diazyme NAG assay uses a specific substrate, 2-methoxy-4-(2’nitrovinyl)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-GlcNAc) that is hydrolyzed by NAG to produce 2-methoxy-4-(2’-nitrovinyl)-phenol product. The product formation is detected at 505 nm upon addition of an alkaline buffer.

 

 

Intended use
The N-acetyl-β-D-glucosaminidase (NAG) assay kit is for the quantitative in vitro determination of NAG in patient urine samples. The assay is for investigational use or export only.
Product Catalog Number Packaging Method/Format
Kit (540 Tests) DZ062A-K R1: 1 x 75 mL
R2: 1 x 15 mL
R3: 1 x 30 mL
Colorimetric, endpoint
Liquid Stable
Calibrator DZ062A-Cal 1 x 2 mL  
Control DZ062A-Con 2 x 2 mL