Product Features
The Diazyme enzymatic homocysteine assay is a convenient dual vial liquid stable assay ideal for analyzers which require two part reagent systems.  The assay provides a fast, accurate cost effective alternative to cumbersome and expensive immunochemical methods. The assay is offered with convenient instrument specific packaging options which eliminate reagent transfer for Olympus AU (400/600/640/680).   Accurate and reliable, the assay features excellent correlation with HPLC and Immunochemical methods with a linear range of 2-50 mmole/L and intra and inter %CV values of  <5%. The assay has no significant interference from lipemic and hemolytic substances and eliminates the “carry-over” interference with iron or lipase reagents that can occur with other methods. More significantly, the assay is not interfered by the endogenous cystathionine and therefore can be used with confidence in patients with renal disease.   Stable for 12 months from date of manufacturer when stored @ 2° - 8° C. 


Assay Principle
The Diazyme enzymatic tHcy assay is based on a novel assay principle that assesses the co-substrate conversion product (a molecule that is not a substrate of the Hcy conversion enzyme, and does not contain any element from the sample Hcy). In the assay, oxidized Hcy is first reduced to free Hcy which then reacts with a co-substrate, SAM, catalyzed by a homocysteine S-methyltransferase. The co-substrate conversion product is amplified by coupled enzymatic cycling reactions. The conversion of Hcy in the sample is indirectly proportional to the amount of NADH conversion to NAD+ (ΔA340nm).

 

Intended use
For the quantitative in vitro determination of total L-Homocysteine in human serum or plasma.


 

Product                

Catalog Number                

Packaging                  

Method/Format

Kit (185 Tests)

DZ568B-K

R1: 2 x 25 mL
R2: 2 x 7 mL

Enzyme Cycling/
Liquid Stable

Calibrator

DZ568B-Cal

3 x 3 mL

 

Control

DZ568B-Con

4 x 3 mL