Product Features
The Diazyme LDL-Cholesterol assay is a direct dual liquid stable reagent system which can be used reliably with fasting and non-fasting specimens. Reagent transfer is eliminated for most chemistry systems with a choice of convenient instrument specific packaging options including Roche Hitachi 917 series, Olympus AU (400/600/640/680), Beckman Synchron CX, LX and DXC. The assay utilizes Diazyme’s proprietary PVS\PEGME coupled technology which provides outstanding performance. The assay features a linear range of up to 250 mg/dL, intra and inter %CV values <2.0 % and less than 10% interference from triglycerides to 1000 mg/dL, ascorbic acid to10 mmol/L, bilirubin to 40 mg/dL, bilirubin conjugate to 30 mg/dL, hemoglobin to 1000 mg/dL. The reagent is stable for 12 months from date of manufacturer when stored @ 2°- 8°C. 

Assay Principle
The assay is based on a modified polyvinyl sulfonic acid (PVS) and polyethylene- glycol ether (PEGME) coupled classic precipitation method with the improvements in using optimized quantities of PVS/PEGME and selected detergents. LDL,VLDL, and chylomicron (CM) react with PVS and PEGME and the reaction results in inaccessibility of LDL, VLDL and CM by cholesterol oxidase (CHOD) and cholesterol esterase (CHER), whereas HDL reacts with the enzymes. Addition of R2 containing a specific detergent releases LDL from the PVS/PEGME complex. The released LDL reacts with the enzymes to produce H2O2 which is quantified by the Trinder reaction. 


 

Intended use
The Diazyme LDL-Cholesterol reagent is intended for the quantitative in vitro determination of Low Density Lipoprotein Cholesterol in human serum or plasma. The reagents can assist in the diagnosis and treatment of patients at risk of developing coronary heart disease. Elevated LDL cholesterol is associated with the increased risk of developing cardiovascular diseases.

 

Product             

Catalog Number             

Packaging             

Method/Format             

Kit (1120 Tests)

DZ128A-K

R1: 4 x 70 mL
R2: 2 x 50 mL

Modified PVS/PEGME/
Liquid Stable

Calibrator

DZ130A-Cal

1 x 1 mL

 

Control

DZ130A-Con

2 x 3 mL