Product Features
The assay provides an extended linear range from 5 to 250 U/L with virtually no interferences from triglyceride (300 mg/dL), hemoglobin (150 mg/dL), and bilirubin up to (20 mg/dL). The Diazyme lipase assay has high precisions with Inter-Run %CV’s of less than 5%, and Intra-Run %CV’s of less than 6% at 215 U/L. The assay has an excellent correlation to Beckman™ lipase reagent with r2 = 0.997. Reagents are stable for 12 months when stored at 2°-8°C. 

Assay Principle
The assay is based on the cleavage of a specific chromogenic lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6’methyl-resorufin)-ester emulsified in stabilized micro-particles. The substrate is converted by lipase into 1,2-O-dilauryl-rac-glycerol and glutaric acid-6’-methylresorufin-ester which decomposes spontaneously to glutaric acid and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample. 


Intended use
The Diazyme Lipase assay is intended for the quantitative in vitro determination of lipase in serum or plasma. Determination of lipase is used for assisting diagnosis of diseases of pancreas such as acute and chronic pancreatitis and obstruction of the pancreatic duct. 

Product             

Catalog Number             

Packaging             

Method/Format             

Kit (300 Tests)

DZ132A-K

R1: 2 x 50 mL
R2: 2 x 11 mL

Colorimetric,
Liquid Stable

Calibrator

DZ132A-Cal

1 x 1 mL

 

Control

DZ132A-Con

2 x 3 mL