The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) exists as several dimeric isoenzymes (αα, αβ, αγ, ββ and γγ) composed of three distinct subunits α, β and γ. The γ-unit is found either in a homologous γγ- or in a heterologous α, γ-isoenzyme and is known as neuron-specific enolase (NSE). The monoclonal antibodies used in the CanAg NSE EIA bind to the γ-subunit of the enzyme and thereby detects both the γγ- and the αγ-forms (1, 2). The NSE levels are low in healthy subjects and subjects with benign diseases. Elevated levels are commonly found in patients with malignant tumours with neuroendocrine differentiation, especially small cell lung cancer (SCLC) (3) and neuroblastoma (4).
Quantitative determination of NSE in serum may be valuable in the management of patients with suspected or diagnosed SCLC or neuroblastoma, to confirm the diagnosis, to monitor the effect of treatment and to aid in the detection of recurrent disease (5, 6).

SPECIFICATIONS

Results within:

2 hours, one step procedure

Detection limit:

< 1 µg/L

Measuring range:

1 - 150 µg/L
(may be extended by sample dilution)

Sample volume:

25 µL

Hook effect:

No hook up to 200 000 µg/L

Stability:

18 months at 2-8°C

Standard range:

0 - 150 µg/L

Incubation temp:

20 - 25°C

Recovery:

96 - 109 %

Detection:

620 nm or 405 nm