CA125 is a high molecular weight mucin type glycoprotein, originally defined by the Oc125 monoclonal antibody (MAb) established by Bast et al. (1). Different epitopes, co-expressed with the Oc125 epitope on the CA125 antigen, have been used for the development of heterologous assays for determination of the CA125 antigen (2). The CanAg CA125 EIA is based on two mouse monoclonal antibodies, Ov197 and Ov185, directed against two independent epitopes of the protein core of the CA125 antigen (3, 4). Assays for CA125 are frequently used to monitor patients with gynecological malignancies such as epithelial ovarian cancer (5).


Results within:

4 hours

Detection limit:

< 1.5 U/mL

Measuring range:

1.5 - 500 U/mL
(may be extended by sample dilution)

Sample volume:

25 µL

Hook effect:

No hook up to 50 000 U/ml


18 months at 2-8°C

Standard range:

0 - 500 U/ml

Incubation temp:

20 - 25°C


101 - 104 %


620 nm or 405 nm


> 0.95 compared with Enzymun-Test CA125 II