Tissue Polypeptide Antigen cyk
TPAcyk is a sensitive indicator of tumour cell activity. It is particularly useful in the management of patients with different epithelial cell carcinomas, in establishing prognosis, in treatment monitoring and in follow-up of patients after concluded therapy.

TPAcyk is a quantitative serum monoclonal immunoassay with reactivity against defined epitope structures on cytokeratins 8 and 18

TPAcyk ELISA is a solid phase sandwich assay based on immunochemical technique. Standards, controls and samples react simultaneously with solid phase catcher antibodies (6D7 and 3F3) and the HRP conjugated detector antibody during incubation in the microstrip wells. After washing, the TMB substrate is added. Subsequently the reaction is stopped and the absorbance is read. The developed colour is directly proportional to the concentration of the analyte.